Azadirachta indica is the most useful medicinal plant in india. Each part of plant has some medicinal property. It is now considered as a valuable source of unique natural products for development of medicines against various diseases. In present study focus on the in vitro hepatoprotective activity of the aqueous and methanolic extract of the leaves of the plant. In present study extract was added to the suspension of freshly isolated rat hepatocytes incubated in the calcium free Hank’s buffer pH 7.4 before the addition of carbon tetrachloride (CCl4). Both the aqueous extract and methanolic extract in (10, 50 and 100 µg/ml) shows protective effect against CCl4 induced cytotoxicity. Hepatocyte exposed to CCl4 the shows reduction of hepatocyte viability. The hepatocyte preincubated with aqueous and methanolic extract shows dose dependant protection from cytotoxicity produced by CCl4. In addition glutamate pyruvate transaminase, alkaline phosphatase, and aspartate transaminase activity in aqueous and methanolic extract were found lesser than that observed with hepatocyte treated with CCl4 alone. Additionally reduced glutathione levels in hepatocyte incubated with aqueous and methanolic extract in different dose were found to be higher than that observed with CCl4 alone. Our result suggested that aqueous and methanolic extract shows hepatoprotective effect in vitro rat hepatocyte.
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